Optimizing NTRK gene fusion detection

NTRK gene fusions are an important class of oncogenes which occur in a wide range of adult and pediatric tumor types.1 Several methods exist to detect genomic alterations. However, features unique to NTRK gene fusions must be considered during testing—making NTRK gene fusion detection an important challenge for pathologists.1,2

Optimizing testing for genomic alterations is the critical first step to identify NTRK gene fusions

This website provides information to help pathologists optimize their NTRK gene fusion testing process.

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Identifying Genomic Alterations

Why Test for Genomic Alterations? Gene Fusions in Cancer

NTRK Gene Fusions in Oncology

What Are Gene Fusions? Role of NTRK Gene Fusions in Oncogenesis NTRK Gene Fusions in Cancer NTRK Gene Fusion Characteristics

Detecting NTRK GENE Fusions

Key Methodologies Overview Guidelines for NTRK Gene Fusion Testing NGS Testing IHC Testing FISH Testing RT-PCR Testing

Optimizing NGS for NTRK Gene Fusions

NGS Overview RNA- vs DNA-based NGS NGS Methodologies NGS Workflow Overview Preanalytic Phase Analytic Phase Postanalytic Phase Choosing a Commercial NGS Kit Reference NGS Laboratories

Laboratory Considerations and Logistics

Key Laboratory Considerations Tissue Sufficiency Across Tumors Sample Preparation Sample Considerations Across Methodologies Turnaround Across Methodologies Batching Considerations Across Methodologies

Resource Library

DNA, deoxyribonucleic acid; FISH, fluorescence in situ hybridization; IHC, immunohistochemistry; NGS, next-generation sequencing; NTRK, neurotrophic tyrosine receptor kinase; RNA, ribonucleic acid; RT-PCR, reverse-transcription polymerase chain reaction.

References:

Amatu A, Sartore-Bianchi A, Siena S. ESMO Open. 2016;1:e000023. Return to content
Vaishnavi A, Le AT, Doebele RC. Cancer Discov. 2015;5(1):25-34. Return to content

Optimizing NTRK gene fusion detection

Optimizing testing for genomic alterations is the critical first step to identify NTRK gene fusions

Anatomical Pathology

Molecular Pathology

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Optimizing NTRK gene fusion detection

Optimizing testing for genomic alterations is the critical first step to identify NTRK gene fusions

Anatomical Pathology

Molecular Pathology

Select a Topic

References: